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Vitiligo Model OR

Vitiligo Model OR

Vitiligo Model

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Vitiligo, an autoimmune pigment-cell disease, is on the rise. The disease is characterized by the development of depigmented white spots on the skin, but the cause, cure, and its long-term impact on patients remain unknown. Researchers at Emory University conducted a study using a new gene-editing technique, CRISPR-Cas9, to deftly remove the faulty Stella Maffulli reported the following latest health-related news from the Ecole Polytechnique Fédérale de La Where Best to Go for Inherited Hair Stuff? A handy guide to developing a hereditary hair loss plan to J. Nathan Haynes, MD New research suggests an underlying genetic pattern that may be a target for treatment.

Skin

By studying two different families with this common disease, scientists may have made a Based on animal experiments, researchers at Aarhus University in Denmark and Yale University in the United States propose that a virus may be involved. "Because Chakras, Ceremonial Use: A Brief History of the Chakra System of Nine Energy Centers Within the Human Body In their simplest Here we show that white spots result from the decreased production of a non-conventional protein called vitiligo protein (VP), a member of the Some researchers have speculated that the most plausible cause of vitiligo, like vitiligo of the albinistic type, is allergic inflammation that attacks

Basic Protocol 1 describes detailed methods for inducing epidermal depigmentation in mice. Alternate Protocol 1 describes a method to screen for potential treatment targets and identify those that prevent vitiligo development. Alternate Protocol 2 describes a method to study disease reversal known as repigmentation and to quantitate it using Image J. Support Protocol 1 describes how skin should be processed to facilitate analysis of skin cells by flow cytometry, while Support Protocol 2 includes methods for the detection of effector CD8 (Source: www.ncbi.nlm.nih.gov)

Model

T cells using a recombinant vaccinia virus that expresses their cognate antigen. The major difference between this model and previous models is the location of depigmentation. C57BL/6 mice have pink skin due to the lack of melanocytes present within the epidermis and most melanocytes reside in the hair follicles (Nishimura et al., 2002). Previous models using C57BL/6 mice displayed only hair depigmentation, which is not representative of human disease, reviewed in Essien & Harris, 2014 and summarized in Table 1. Here, we utilize mice that retain melanocytes in the epidermis due to transgenic expression of a melanocyte growth factor, resulting in black skin and black hair (Kunisada et al., 1998) (Figure 1). Induction of vitiligo in these Stem Cell Factor (SCF) transgenic mice leads to epidermal depigmentation (Figure 2A,B) that spares melanocytes in the hair follicle, similar to human vitiligo. Because of the sparing of these follicular melanocytes, mice in this model are capable of perifollicular repigmentation, similar to how disease is reversed in humans. Induction of vitiligo in mice is dependent on three parameters: sublethal irradiation, transfer of melanocyte-specific CD8

Each skin site is awarded a score between 0–5; a score of 0 reflects no evidence of depigmentation, and a score of 5 reflects 100% depigmentation at that site. Thus, the highest score each mouse can achieve is 20. For unbiased scoring of vitiligo mice, a blinded but trained investigator who is not aware of the experimental set up details should score the mice. (Source: www.ncbi.nlm.nih.gov)

 

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