Add your company website/link
to this blog page for only $40 Purchase now!Continue
FutureStarrLicorice Planta Medicinal
To validate the HRM results, we performed capillary electrophoresis on heat denatured PCR products labeled with FAM. These results revealed that each Glycyrrhiza species had distinct patterns and that the five species could be differentiated from each other. This demonstrates that the newly designed SSR primers can be used for licorice species identification (Figure 3 and Supplementary Figure S2) and is consistent with our HRM results. Interestingly, analysis of sequenced DNA found that some amplicons not only exhibited different microsatellite alleles, but also contained single nucleotide polymorphisms (SNPs) in regions flanking the SSR (data not shown here due to the poor quality of SSR sequencing). The presence of SNPs in these flanking sequences has been previously reported in other species (Distefano et al., 2012). HRM is a powerful method that can be used to detect SNPs, which means that relative to capillary electrophoresis, its higher sensitivity means that it is better able to discriminate PCR products of the same size that have different base compositions.
This study is the first to use SSR-HRM to detect the species composition in currently available licorice herbal supplements. Our results indicated that genomic DNA can be obtained from medicinal parts (i.e., dried roots) as well as from their processed or modified forms (i.e., decoction pieces or powder), and its quality was suitable for HRM analysis. We used 161 voucher samples to verify the reliability of the newly developed SSR markers. SSR patterns obtained from polyacrylamide gel and HRM analyzes were similar, which indicated that these SSR markers were able to accurately distinguish between the five species. Therefore, we speculate that the SSR markers developed in this study have the power to identify the original plant species used in commercial Glycyrrhiza herbal supplements as well as their substitutes.
Plants have always been an important source of medicines for humans, and licorice is a very significant herb in the development of humans. As a traditional herb, it is widely cultivated in China, Japan, Russia, Spain and India. With the development of organic chemistry and biochemistry, various chemical ingredients extracted from licorice have been studied and identified. Among them, many chemical components were considered to have strong pharmacological activities, such as anti-inflammatory, anti-ulcer, antibacterial, anticancer and so on. Based on those reports, licorice has attracted the attention of many researchers in recent years, and they are devoted to discovering the active ingredients and mechanism of action of active compounds. Licorice flavonoids are one of the main extracts of licorice root and stem and have many potential biological properties. This paper aims to summarize the four kinds of licorice flavonoids, including liquiritigenin, isoliquiritigenin, licochalcone (including licochalcone A and licochalcone B) and glabridin, about their biological activities of anti-inflammatory, anticancer, antibacterial.Hsu, Y.L.; Wu, L.Y.; Hou, M.F.; Tsai, E.M.; Lee, J.N.; Liang, H.L.; Jong, Y.J.; Hung, C.H.; Kuo, P.L. Glabridin, an isoflavan from licorice root, inhibits migration, invasion and angiogenesis of MDA-MB-231 human breast adenocarcinoma cells by inhibiting focal adhesion kinase/Rho signaling pathway. Mol. Nutr. Food Res., 2011, 55(2), 318-327. (Source: www.eurekaselect.com)